LC run time (min) | Acetonitrile in presence of (0.1% Formic acid) | Water (%) | ||
---|---|---|---|---|
0 | 60 | 40 | ||
3 | 100 | 0 | ||
4 | 100 | 0 | ||
5 | 60 | 40 | ||
10 | 60 | 40 | ||
Retention time (min) | Lower Limit of Detection (ng) | Flow rate (μL/min) | Injection volume (μL) | Column Temperature (°C) |
1.92 | 0.5 | 200 | 3.0 | 45 |